Clinical Case: A 77-year-old man with a history of hypertension (stage 2) and multiple phalanx amputations of the right hand due to Raynaud's disease, referred to our regenerative medicine Clinic for a cutaneous ulceration (of arterial origin) of the right lateral malleolus (size: 4x3 cm), that had previously been refractory to a 3-year conventional medical (topical) treatment prescribed by a dermatologist. The patient showed a complete healing of the ulcer after five injections of hyperconcentrated platelet-rich plasma (High-PRP).

Materials and Methods: For each application of High-PRP, the extraction of 51 ml of blood was carried out aseptically using the closed vacuum system with sterile tubes for humans (BD) with ACD Solution A. Centrifugation was performed for 48 minutes (according to the Stem Cell Therapy Argentina ^® private protocol) to obtain a hyperconcentrated Pure Plasma Platelet product (an average concentration of 1,900,000 platelets/ml (± 400,000).

Results: Overall, 5 injections of High-PRP (3 ml per each injection) were administered 30 days apart. Each 3 ml injection consisted of 10 consecutive injections (0.3 ml per each), administered by clockwise rotation, with a 3 ml luer lock syringe and a 30G needle, without local anesthesia. After 180 days, the ulcer was completed closed.

Conclusions: PRP could be considered as a safe and cost-effective treatment for cutaneous wounds, aimed to promote the healing process and shorten wound healing time. Therefore, the use of PRP can be considered as a valid option for the treatment of chronic wounds of different etiologies.