The skin aging and the loss of volume are the most involved processes in facial aging; the injection treatment with viable adipocytes and Adipose-Derived Stromal and stem Cells (ADSC) promises to restore the volume and regenerate the skin. To achieve a higher adipocytes engrafting rate and a skin regenerative effect by ADSC the small tissue clusters must be injected superficially into the dermal and subdermal layers. The Authors describe SEFFI and MicroSEFFI techniques which through an innovative harvesting procedure provide three different kinds of injectable tissue with tissue clusters of different size: these three different tissues are injected in the dermal and subdermal layers of the different face areas that have different skin and subdermal thickness thus minimizing the risks of visibility and lumpiness.